2024 Glut4 antibody cell signaling

Glut4 antibody cell signaling

Author: gheb

August undefined, 2024

WebSep 4, 2024 · e Co-immunoprecipitation assay for interaction between endogenous PGRMC1 and GLUT4. 3T3L1 cell lysate was incubated with anti-PGRMC1 antibody or normal rabbit IgG, and then incubated with 10 µl ... WebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab216661) Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat …

Insulin can regulate GLUT4 internalization by signaling to Rab5 ... - PNAS

WebGlucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded, in humans, by the SLC2A4 … WebCell Signaling Technology. 3 Trask Lane. Danvers, MA 01923. [email protected]. www.cellsignal.com. 8776162355. headquarters: USA. Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help ... filtering crucible

Glut4 (1F8) Mouse mAb from Cell Signaling Technology

WebGLUT4 Antibody (PA5-23052) in ICC/IF. Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase 3T3-L1 cells differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. WebOct 14, 2016 · Anti-phospho-Akt (Ser-473) antibody and anti-Akt antibody were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-FLAG antibody and anti-FLAG antibody-conjugated agarose … WebJun 14, 2013 · Membranes were stained with anti-GLUT4 antibody (C-20 goat polyclonal antibody, Santa Cruz Biotech), total Akt antibody (Cell Signaling), phospho-CREB … filtering cooking oil paper towel

Recombinant Anti-Glucose Transporter GLUT4 (phospho …

GLUT4 Rabbit Polyclonal Antibody (GLUT4 兔多抗)(AF6999)

Web3T3-L1 prepreadipocytes were cultured in DMEM containing 25 m m glucose, 10% fetal serum at 37 °C, and 5% CO 2.L6 muscle cells expressing c-myc epitope tagged GLUT4 (L6myc cells) were maintained in myoblast monolayer culture in α-MEM containing 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic solution (100 units/ml penicillin G, 10 μg/ml … WebGlut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and ... growth australia abnWebAll lanes : Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml Lane 1 : Recombinant Protein GLUT4 (Partial, Tagged) at 0.1 µg Lane 2 … filtering csv data in python

"WebJul 25, 2024 · In order to assess the effects of n-3PUFA on fatty acid metabolism and insulin sensitivity in muscle cells, Cell Signaling Technology GLUT4 antibody (Cell Signaling Technology, 2213) was used in western blot on mouse samples (fig 1). Biochim Biophys Acta (2016) ncbi. mouse monoclonal (1F8) 2213. " - Glut4 antibody cell signaling

Glut4 antibody cell signaling

Neferine Promotes GLUT4 Expression and Fusion With the Plasma …

WebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created … WebAll lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution Lane 1 : Human fetal heart whole cell lysate Lane 2 : Human fetal heart whole cell lysate with Phosphatase Lysates/proteins at 15 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 …

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Web蛋白描述: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake By similarity. Low affinity receptor which can bind to NGF, BDNF, NT-3 ... WebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin … Monoclonal Antibody for studying Glut4. Cited in 185 publications. Validated for …

WebThe anti-GLUT4 antibody was obtained from Abcam (Cambridge, UK). The Anti-GAPDH rabbit polyclonal antibody and the high sensitivity chemiluminescence detection kit and BCA protein assay kit were purchased from Kangwei Century Biology Co., Ltd. (Beijing, China). Goat anti-Rabbit IgG was provided by Lianke Bio Company (Hangzhou, China). WebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles …

WebCell Signaling Technology Inc mouse anti glut4 monoclonal antibody Mouse Anti Glut4 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various … WebAll lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution Lane 1 : Human fetal heart whole cell lysate Lane 2 : …

WebAbcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. ... Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to ...

WebOct 30, 2001 · The increased cell-surface GLUT4 level in the Rab5 antibody-injected cells could be caused by either a decrease in the rate of the GLUT4 internalization or a delay in GLUT4 internalization, or both. To test specifically the effect of Rab4 and Rab5 on GLUT4 internalization, 3T3-L1 adipocytes were stimulated with 1.7 nM insulin for 20 min. filtering cooking oilWebThe Glut4 (1F8) Mouse mAb Antibody from Cell Signaling Technology is a Mouse Monoclonal antibody to SLC2A4. This antibody recognizes Human, Mouse, and Rat … growth award namesWeb(2213) Glut4 (1F8) Mouse mAb - Cell Signaling Technology - CiteAb. 188 citations – A mouse monoclonal antibody, raised against Solute carrier family 2, facilitated glucose transporter member 4 (Human), supplied by Cell Signaling Technology. ... Monoclonal antibody is produced by immunizing animals with partially purified intracellular glucose ... filtering creek waterWebInsulin signaling pathway. Our poster covers glucose interaction with GLUT-4 and the downstream signaling pathways, as well as insulin binding to insulin receptors and the downstream effects of this. Insulin is a peptide hormone synthesized by pancreatic beta cells located in the islets of Langerhans. Secreted in response to elevated levels of ... filtering cordless paper pleated shadeWebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created a. ... and anti-phospho-ATF-2 (Thr 71) polyclonal antibody from Cell Signaling Technology (Beverly, MA). A polyclonal anti-GLUT4 rabbit antibody was raised in our laboratory to a ... growth automobile industryWebWestern Blot: Glut4 Antibody [NBP1-49533SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in … filtering criteriaWebInsulin Signaling; Warburg Effect; AMPK Signaling ... Western blot analysis of extracts of various cell lines, using GLUT4 antibody at 1:1000 dilution. Secondary antibody: HRP … growth at side of the toe