M13 intergenic region
WebAssembled from M13 and GenBank:ECOLCM13D by F. Pfeiffer The lac region was cloned into another site of the M13 intergenic region, compared to the M13mp series. The PiVX polylinker was cloned into mWB2342 to construct mWB2344. The BamHI site in the polylinker region is not unique, because the BamHI site at pos 2220 of phage M13 has … WebConstructed using M13 replication and kanR selection mechanisms from pUK21, T7 regulatory sequences from a 271 bp BglII-EcoRV fragment of pET3b and leader peptide sequence and multiple cloning sites from pEZZ18. Mycoplasma contamination Not detected Vector information Construct size (kb) 3.38100004196167 Intact vector size 3.381 Vector …
M13 intergenic region
Did you know?
WebM13 and f1 sequences are slightly different from fd. They both have only 6407 nucleotides; f1 differs from fd in 180 positions (only 10 of these changes are reflected in amino-acid changes in gene products) [16] and M13 has only 59 nucleotide differences from f1. For many purposes the phages in the Ff group can be considered as interchangeable. WebThe M13 file extension indicates to your device which app can open the file. However, different programs may use the M13 file type for different types of data. While we do not …
WebMar 17, 2024 · Most of what is known about filamentous phage assembly comes from work on E. coli bacteriophage Ff (f1, M13 and fd) which are 98% identical at the DNA sequence level and have been studied interchangeably [ 97, 98, 106, 121, 134 ]. WebThe wild-type M13 genome is 6407 bp encoding ten genes essential for its life cycle. However only 507 bp of the genome, termed the intergenic region, is not …
WebThe genomes of the filamentous bacteriophages f1, fd and M13 have been completely sequenced and shown to code for 11 proteins (Fig. 2). There is an intergenic region of approximately 500 nucleotides which contains the origins of replication for the viral and complementary DNA as well as the packaging signal needed for efficient assembly. WebAug 25, 1984 · The origin of replication for the viral strand of bacteriophage M13 DNA is contained within a 507 base-pair intergenic region of the phage chromosome. The viral …
WebCC plasmids containing M13 intergenic region which preferentially CC packages single-stranded plasmid DNA over phage DNA. (ATCC staff) CC Restriction digests analyzed on agarose gels give the following sizes CC (kb): BamHI--8.7; HindIII--8.7. (ATCC staff) CC Medium is 1592 SM buffer. CC NM (M13K07) CC CM (no) CC NA (ds-DNA)
WebM13 Intergenic Region: Start_codon: No: Further_information: pZ150 contains the M13 IG fragment; the M13 intergenic region is thought to contain a cis-acting morphogenic signal for packaging and orientation of the DNA within the phage particles: Price_code: 1 sylvie facon creatrice franceWebAug 25, 1984 · The origin of replication for the viral strand of bacteriophage M13 DNA is contained within a 507 base-pair intergenic region of the phage chromosome. The viral … sylvie ecker thionvilleWebNov 16, 2024 · The M13 pII protein is required to initiate rolling circle replication. We replaced the promoter driving the pII gene with the araBAD promoter, which intrinsically regulates the arabinose operon [ 30 ]. We also eliminated the packaging signal in the M13 intergenic sequence to reduce the contamination by the phage-derived ss DNA [ 31, 32 ]. sylvie facheWebThe insertion point has been localized at 0.083 map unit on thewild-type circular map-i.e., within the intergenic region. The results prove that part of the intergenic region is nonessential and that the phage can be used as a cloning vehicle. tfwm bus service changesWebThe genomes of the filamentous bacteriophages f1, fd and M13 have been completely sequenced and shown to code for 11 proteins ( Fig. 2 ). There is an intergenic region of approximately 500 nucleotides which contains the origins of replication for the viral and complementary DNA as well as the packaging signal needed for efficient assembly. tfwm bus routesWebIG region: intergenic region, usually contains the exposed to an antigen. Useful in analyzing natural humoral responses, for example, in patients with autoimmune disease, viral infection, neoplastic diseases, etc. 20 amino acids) Antibody Formats Fab The light chain (VL-CL) and the Fd-domain (VH-CH1) of the heavy chain of an antibody. tfwm bus timetablesWebM13K07 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … sylvie fashion 54