WebbThe EasySelect Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris. The EasySelect Kit contains Pichia yeast strains, expression vectors, and reagents that enable EasySelect™ 毕赤酵母表达试剂盒 You need to enable JavaScript to run this app. Thermo Fisher Scientific Logo 登录 没有账 … Webb7 juli 2010 · Pichia Expression Kit (Cat. no. K1740-01) available from Invitrogen. Additional general information about recombinant protein expression in Pichia pastoris is provided …

pPICZ A, B, and C - Thermo Fisher Scientific

Webb7 juni 2010 · If you do not have access to an electroporation device, use the LiCl protocol on page 24 or the Pichia EasyComp™ Transformation Kit available from Invitrogen (see below). Pichia If you wish to perform chemical transformation of your Pichia strain with pPIC6 EasyComp™ A, B, or C, the Pichia EasyComp™ Transformation Kit is available … WebbPichia pastoris competent cells preparation by Pichia EasyComp Kit (Invitrogen) Competent cells of Pichia pastoris were obtained by first mixing 10 ml of YPD with a single colony of X-33. It was grown overnight in a shaking incubator at 28-30 oC. The cells were then diluted in 10 ml of YPD until it reached an OD600 of 0.1-0.2. may we have your confirmation

Expression and Characterization of a Recombinant …

Webbthe Pichia EasyComp Kit, and pPICZaA vector were purchased from Invitrogen (Carlsbad, CA). Restriction enzymes were purchased from Fermentas (Hanover, MD). Carboxymethyl cellulose (CMC) and osta-zin brilliant red-hydroxyethyl cellulose (OBR-HEC) were purchased fromSigmaChemical(St.Louis,MO). WebbThe Pichia EasyComp™ Transformation Kit contains sufficient reagents (Solutions I, II, and III) for 6 preparations of competent cells. Each competent cell preparation yields enough … WebbOriginal Pichia Expression Kit 1 kit K1710-01 EasySelect™ Pichia Expression Kit 1 kit K1740-01 Pichia EasyComp™ Transformation Kit 1 kit K1730-01 Pichia Protocols 1 book G100-01 ® One Shot TOP10 20 reactions C4040-03 (chemically competent cells) One Shot® TOP10 Electrocomp™ 20 reactions C4040-52 (electrocompetent cells) may we have the future