Psicheck vector
WebSpecimen collection. The samples from 95 ovarian cancer patients were collected from 2012 to 2024. The diagnosis of all patients was confirmed by surgery and pathology results in the Shengjing Hospital of China Medical University, and all patients only received PTX-based chemotherapy after surgery, the written informed consent form was obtained prior … WebDual-luciferase reporter assay The psiCHECK-2 vector ( Promega, Cat # C8021) was used to construct plasmids for dual-luciferase reporter assay. RNF146 3′UTR or its mutant was cloned October 12, 2024 Zhaowei Wang, Xiaoling Xia, Jiaqi Li, Tatsushi Igaki
Psicheck vector
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WebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the …
Web2 days ago · The luciferase assay was performed with the Dual-Luciferase Reporter Assay System (Promega). WT and mutant PABPC1L 3′UTR were cloned into the C-terminus of the Renilla luciferase gene in the psiCHECK™-2 vector. Empty vector was used as the control, and Firefly luciferase expression served as the internal standard for transfection efficiency. WebJul 19, 2012 · More importantly, psicheck-hs-146b-5p could not be affected by hsa-miR-146b-3p mimics and another unrelated miRNA hsa-miR-218 could not inhibit Renilla luciferase activity expressed from psicheck-hs-146b-3p . This indicated that the decreased luciferase signal was specific for the activity of the corresponding miRNA.
WebDec 10, 2012 · To quantitatively determine the extent of repression, the expression of Renilla luciferase ( Rluc) from the psiCHECK-2 vector (psiCHECK) was used as a spliced mRNA model, through the introduction of a chimeric intron that was originally inserted to augment expression ( Fig. 1A ). WebThe psiCHECK™-1 Vector(a–d) (Cat.# C8011) and psiCHECK™-2 Vector(a–f) (Cat.# C8021) are designed to provide a quantitative and rapid approach for optimization of RNA …
WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target …
http://www.biovector.net/product/44170.html toy stores in tampa floridaWebMCF-7 cells were transfected with a reporter vector psiCHECK2-FSCN1 3'UTR or psiCHECK2 control vector and miR-133a mimic or negative control. Each transfection was carried out in triplicate. Luciferase assay was performed 48 h after gene transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. toy stores in south africaWebIn this study, we replaced the SV40 promoter in the psiCHECK-2 reporter vector, which is widely used with mammalian cell lines, with the HSV-TK or AC5.1 promoter to yield two … thermomix emailWebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECK TM-2 vector, we found that seven of the eight sites in the Col4a3 – Col4a6 gene complementary to miR-29 were … thermomix en amazonWebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table … thermomix endivesWebVector backbone psiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Vector type Luciferase Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name 3 miR-122 target sites Insert Size … thermomix emploihttp://www.honorgene.com/uploads/files/202404/5ea518d17dd8f.pdf thermomix empik