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Stat3 phospho-y705 kit

WebProduct Description. CST's PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … WebQuality Control: the Phospho-STAT3 (Y705) + Total STAT3 assay kit is routinely tested against IFNα-treated HeLa lysates. HeLa cells were cultured in a T175 flask to 90% confluence and stimulated with 3 nM of IFNα for 30 min at RT. Following cell lysis using 4 mL of 1X Lysis Buffer 1,

STAT3 (phospho Y705) peptide (ab179551) Abcam

WebPhospho-Stat3-Y705 Antibody kit (RK04099) Datasheet Tested applications: Reactivity: Human, Mouse Datasheet Reviews Protocols Inquire About This Product Overview Product Component Background The protein encoded by this gene is … WebApr 12, 2024 · Y705-STAT3 and p65 acted additively in reducing CSC enrichment, and Y705A-STAT3 variant + sip65 has enriched chemo-resistant CSCs. ... Millipore, Temecula, CA, USA) was added. For IL-6 and IFNγ, the following ELISA kits were used: IL-6 (#900-TM16; Peprotech), IFNγ (#900-TM27, Peprotech). The reaction was stopped by ... “High P-Y705 … free clinic long beach ca https://ajrnapp.com

Phospho-Stat3 (Tyr705) Antibody Cell Signaling Technology

WebSummary for Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA. This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human and mouse STAT3 (Y705) in cell lysates. An immobilized capture antibody specific for STAT3 binds both phosphorylated and unphosphorylated … WebSTAT3 phospho Y705 Antibody Rabbit Polyclonal Item No. 600-401-C64 Size: 100 µg Applications: ELISA, IHC, WB Reactivity: Human Host Species: Rabbit Have a Question? Ask a Specialist CONTACT US OR +1 484.791.3823 $369.00 /Per Item 25 µL $125.00 100 µg $369.00 Add To Cart Add to List Bulk Orders Availability: Ships next business day … WebSTAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by … free clinic meadville pa

检测培养细胞系中磷酸化-STAT1（pTyr(pTyr 701 ),磷酸化-STAT3 …

Category:Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), APC …

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Stat3 phospho-y705 kit

STAT3 (Phospho) [pY705] Multispecies ELISA Kit - Invitrogen

WebJun 25, 2016 · Antibodies phospho-STAT3(Y705), STAT3, cleaved caspase-3, cleaved PARP, phospho-p65, phospho-STAT1 (Y701), STAT1, phospho-JAK2 (Y1007/1008), JAK2, phospho- JAK1 (Y1022/1023), JAK1, phosphor-JNK (Y183/Y185), phosphor-ERK (T202/Y204), GAPDH HRPconju- gated secondary antibodies were from Cell Signaling Technology (Beverly, … http://resources.perkinelmer.com/lab-solutions/resources/docs/MAN_LANCE_Ultra_pSTAT3_TRF4004.pdf

Stat3 phospho-y705 kit

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http://sigma-aldrich.cnreagent.com/pdetail407557.html WebDec 21, 2012 · Five hundred nanograms of DNA were bisulfite-converted with the Epitect Bisulfite Kit (Qiagen) according to the manufacturer's instructions. ... phospho-STAT3 …

WebSTAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon … WebJun 25, 2016 · Antibodies phospho-STAT3(Y705), STAT3, cleaved caspase-3, cleaved PARP, phospho-p65, phospho-STAT1 (Y701), STAT1, phospho-JAK2 (Y1007/1008), JAK2, …

WebWestern blot was performed and showed that the phosphorylation level of STAT3 Y705 was decreased in T2DM mice and notably increased in Rb1- or Met-treated T2DM mice (Fig. 5 … WebApr 14, 2024 · Phospho-STAT3 (Y705) Antibody - Prepare a 100X working concentration of the antibody by reconstituting with 110 L of 1X PBS. After reconstitution, store at 2-8° C …

WebNo lysate preparation required. Results with as few as 10,000 cells per well. Measure two proteins simultaneously in the same well. Ideal for measuring phosphorylation. Design allows for normalization of well-to-well variations. Complete kits require minimal optimization. Suitable for high-throughput analysis of non-adherent cells.

WebPhospho-STAT3 (Tyr705) Antibody (17-9033-41) in Flow Intracellular staining of untreated (left) or 15-minute IL-6-treated (right) normal human peripheral blood cells with Anti-Human CD4 PerCP-eFluor® 710 (Product # 46-0047-42) and Anti-Human/Mouse phospho-STAT3 (Y705) APC. Cells in the lymphocyte gate were used for analysis. free clinic monroe laWebTHUNDER™ Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cell Signaling Assay Kit. Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 … free clinic midwest city okWebApr 12, 2024 · Y705-STAT3 and p65 acted additively in reducing CSC enrichment, and Y705A-STAT3 variant + sip65 has enriched chemo-resistant CSCs. ... Millipore, Temecula, … blogs about apple macbook airWebCST's PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit #7300. Capture and Detection antibodies (100X stock) and HRP-Linked Secondary Antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. free clinic missouri city txWebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. free clinic martin countyWebPhospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins … blogs about beauty innerWebPhospho-STAT3-Y705 Rabbit mAb Catalog No.: AP0705 Recombinant 15 Publications Basic Information Observed MW 79KDa/88KDa Calculated MW 83kDa/87kDa/88kDa Category ... Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug Phospho-STAT3-Y705 blogs about black and white photography